EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT

The objective of this study was to determine the effect ethanol extract neem (Azadirachta indica) leaves on number spermatids and androgen receptor expression in testicular tissue New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 P3 200 BW 300 respectively. administered orally using a gastric tube for 52 days. At end treatment, terminated testes collected be processed histological preparations stained Periodic Acid Schiff (PAS) staining detect spermatid Immunohistochemical (IHC) expression. results showed that at significantly decreased myoid peritubular cells connective P0.05), but not different Leydig blood vessels (P0.05) tissue. conclusion is dose can used as contraceptive candidate compared BW.